Neurobiology and Computation & Neural Systems seminar.

Neurobiology and Computation & Neural Systems seminar (NB/CNS Seminar)

Monday, October 24, 2022 – 3:30 pm

Broad 100

Yoav Adam

Assistant Professor

The Hebrew University of Jerusalem

Title: All-optical interrogation of hippocampal circuits using voltage imaging

Abstract: Technology for simultaneous control and readout of the membrane potential of multiple neurons in behaving animals will have a high impact on neuroscience research. We developed improved near-infrared voltage indicators, high-speed microscopes, and targeted gene expression schemes that enabled simultaneous in vivo recordings of supra- and subthreshold voltage dynamics in multiple neurons in the hippocampus of behaving mice. The reporters revealed subcellular details of back-propagating action potentials and correlations in subthreshold voltage between multiple cells. In combination with stimulation using optogenetics, the reporters revealed changes in neuronal excitability that were dependent on the behavioral state, reflecting the interplay of excitatory and inhibitory synaptic inputs. In this talk, I will describe the technology, and discuss its limitations and the opportunities it opens. In addition, I will present our current efforts to use all-optical electrophysiology for the detailed dissection of hippocampal circuits during spatial navigation tasks.